buffer b Search Results


alpha sure fire elisas  (Revvity)
91
Revvity alpha sure fire elisas
Alpha Sure Fire Elisas, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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buffer b  (ADS Biotec)
94
ADS Biotec buffer b
Buffer B, supplied by ADS Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
buffer b - by Bioz Stars, 2026-03
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buffer b  (Eppendorf AG)
99
Eppendorf AG buffer b
Phospholipids affect PLIN2 binding to adiposomes Adiposomes were prepared using different amount of PtdIns or DOPE with DOPC in molar ratio. And then, the size of these adiposomes were measured using dynamic light scattering (DLS) (Delsa Nano C Particle Analyzer, Beckman Coulter). After preparation, adiposome preparation (OD600 = 20) was aliquoted equally to 30 μl each and incubated with 1.2 μg PLIN2 per aliquot, supplementing corresponded volume of <t>buffer</t> <t>B</t> to prepare an equal 30 μl specimen. After incubation, the adiposomes were reisolated and washed to remove nonspecific binding proteins. The adiopsome-bound PLIN2 was determined using Western blot with anti-PLIN2 antibody and the density of protein band was quantified by ImageJ. (A) The average diameters of adiposomes with dose of PtdIns (from 0 to 9.6%, mol/mol). (B) The average diameters of adiposomes with dose of DOPE (from 0 to 19%, mol/mol). Inputs of 7.6% of PtdIns and 19% of DOPE were corresponded to the physiological ratio of the two phospholipids on LDs ( Bartz et al. , 2007a ). (C) The binding of PLIN2 on adiposomes with increased doses of PtdIns. (a) The Western blot. (b) The statistical analysis of Western blots using ImageJ. (D) The binding of PLIN2 on adiposomes with increased doses of DOPE. (a) The Western blot of PLIN2. (b) The statistical analysis of Western blots using ImageJ. Data represent mean ± s.e.m., n = 3. ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001, two-tailed t-test. See also <xref ref-type=Figure S2 and . " width="250" height="auto" />
Buffer B, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/buffer b/product/Eppendorf AG
Average 99 stars, based on 1 article reviews
buffer b - by Bioz Stars, 2026-03
99/100 stars
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buffer a  (Promega)
90
Promega buffer a
Phospholipids affect PLIN2 binding to adiposomes Adiposomes were prepared using different amount of PtdIns or DOPE with DOPC in molar ratio. And then, the size of these adiposomes were measured using dynamic light scattering (DLS) (Delsa Nano C Particle Analyzer, Beckman Coulter). After preparation, adiposome preparation (OD600 = 20) was aliquoted equally to 30 μl each and incubated with 1.2 μg PLIN2 per aliquot, supplementing corresponded volume of <t>buffer</t> <t>B</t> to prepare an equal 30 μl specimen. After incubation, the adiposomes were reisolated and washed to remove nonspecific binding proteins. The adiopsome-bound PLIN2 was determined using Western blot with anti-PLIN2 antibody and the density of protein band was quantified by ImageJ. (A) The average diameters of adiposomes with dose of PtdIns (from 0 to 9.6%, mol/mol). (B) The average diameters of adiposomes with dose of DOPE (from 0 to 19%, mol/mol). Inputs of 7.6% of PtdIns and 19% of DOPE were corresponded to the physiological ratio of the two phospholipids on LDs ( Bartz et al. , 2007a ). (C) The binding of PLIN2 on adiposomes with increased doses of PtdIns. (a) The Western blot. (b) The statistical analysis of Western blots using ImageJ. (D) The binding of PLIN2 on adiposomes with increased doses of DOPE. (a) The Western blot of PLIN2. (b) The statistical analysis of Western blots using ImageJ. Data represent mean ± s.e.m., n = 3. ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001, two-tailed t-test. See also <xref ref-type=Figure S2 and . " width="250" height="auto" />
Buffer A, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/buffer a/product/Promega
Average 90 stars, based on 1 article reviews
buffer a - by Bioz Stars, 2026-03
90/100 stars
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single-strength buffer b  (Fisher Scientific)
90
Fisher Scientific single-strength buffer b
Phospholipids affect PLIN2 binding to adiposomes Adiposomes were prepared using different amount of PtdIns or DOPE with DOPC in molar ratio. And then, the size of these adiposomes were measured using dynamic light scattering (DLS) (Delsa Nano C Particle Analyzer, Beckman Coulter). After preparation, adiposome preparation (OD600 = 20) was aliquoted equally to 30 μl each and incubated with 1.2 μg PLIN2 per aliquot, supplementing corresponded volume of <t>buffer</t> <t>B</t> to prepare an equal 30 μl specimen. After incubation, the adiposomes were reisolated and washed to remove nonspecific binding proteins. The adiopsome-bound PLIN2 was determined using Western blot with anti-PLIN2 antibody and the density of protein band was quantified by ImageJ. (A) The average diameters of adiposomes with dose of PtdIns (from 0 to 9.6%, mol/mol). (B) The average diameters of adiposomes with dose of DOPE (from 0 to 19%, mol/mol). Inputs of 7.6% of PtdIns and 19% of DOPE were corresponded to the physiological ratio of the two phospholipids on LDs ( Bartz et al. , 2007a ). (C) The binding of PLIN2 on adiposomes with increased doses of PtdIns. (a) The Western blot. (b) The statistical analysis of Western blots using ImageJ. (D) The binding of PLIN2 on adiposomes with increased doses of DOPE. (a) The Western blot of PLIN2. (b) The statistical analysis of Western blots using ImageJ. Data represent mean ± s.e.m., n = 3. ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001, two-tailed t-test. See also <xref ref-type=Figure S2 and . " width="250" height="auto" />
Single Strength Buffer B, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/single-strength buffer b/product/Fisher Scientific
Average 90 stars, based on 1 article reviews
single-strength buffer b - by Bioz Stars, 2026-03
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wash buffer b  (LGC Biosearch)
90
LGC Biosearch wash buffer b
Phospholipids affect PLIN2 binding to adiposomes Adiposomes were prepared using different amount of PtdIns or DOPE with DOPC in molar ratio. And then, the size of these adiposomes were measured using dynamic light scattering (DLS) (Delsa Nano C Particle Analyzer, Beckman Coulter). After preparation, adiposome preparation (OD600 = 20) was aliquoted equally to 30 μl each and incubated with 1.2 μg PLIN2 per aliquot, supplementing corresponded volume of <t>buffer</t> <t>B</t> to prepare an equal 30 μl specimen. After incubation, the adiposomes were reisolated and washed to remove nonspecific binding proteins. The adiopsome-bound PLIN2 was determined using Western blot with anti-PLIN2 antibody and the density of protein band was quantified by ImageJ. (A) The average diameters of adiposomes with dose of PtdIns (from 0 to 9.6%, mol/mol). (B) The average diameters of adiposomes with dose of DOPE (from 0 to 19%, mol/mol). Inputs of 7.6% of PtdIns and 19% of DOPE were corresponded to the physiological ratio of the two phospholipids on LDs ( Bartz et al. , 2007a ). (C) The binding of PLIN2 on adiposomes with increased doses of PtdIns. (a) The Western blot. (b) The statistical analysis of Western blots using ImageJ. (D) The binding of PLIN2 on adiposomes with increased doses of DOPE. (a) The Western blot of PLIN2. (b) The statistical analysis of Western blots using ImageJ. Data represent mean ± s.e.m., n = 3. ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001, two-tailed t-test. See also <xref ref-type=Figure S2 and . " width="250" height="auto" />
Wash Buffer B, supplied by LGC Biosearch, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wash buffer b/product/LGC Biosearch
Average 90 stars, based on 1 article reviews
wash buffer b - by Bioz Stars, 2026-03
90/100 stars
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wash buffer b  (Biosearch Technologies Inc)
90
Biosearch Technologies Inc wash buffer b
Phospholipids affect PLIN2 binding to adiposomes Adiposomes were prepared using different amount of PtdIns or DOPE with DOPC in molar ratio. And then, the size of these adiposomes were measured using dynamic light scattering (DLS) (Delsa Nano C Particle Analyzer, Beckman Coulter). After preparation, adiposome preparation (OD600 = 20) was aliquoted equally to 30 μl each and incubated with 1.2 μg PLIN2 per aliquot, supplementing corresponded volume of <t>buffer</t> <t>B</t> to prepare an equal 30 μl specimen. After incubation, the adiposomes were reisolated and washed to remove nonspecific binding proteins. The adiopsome-bound PLIN2 was determined using Western blot with anti-PLIN2 antibody and the density of protein band was quantified by ImageJ. (A) The average diameters of adiposomes with dose of PtdIns (from 0 to 9.6%, mol/mol). (B) The average diameters of adiposomes with dose of DOPE (from 0 to 19%, mol/mol). Inputs of 7.6% of PtdIns and 19% of DOPE were corresponded to the physiological ratio of the two phospholipids on LDs ( Bartz et al. , 2007a ). (C) The binding of PLIN2 on adiposomes with increased doses of PtdIns. (a) The Western blot. (b) The statistical analysis of Western blots using ImageJ. (D) The binding of PLIN2 on adiposomes with increased doses of DOPE. (a) The Western blot of PLIN2. (b) The statistical analysis of Western blots using ImageJ. Data represent mean ± s.e.m., n = 3. ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001, two-tailed t-test. See also <xref ref-type=Figure S2 and . " width="250" height="auto" />
Wash Buffer B, supplied by Biosearch Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wash buffer b/product/Biosearch Technologies Inc
Average 90 stars, based on 1 article reviews
wash buffer b - by Bioz Stars, 2026-03
90/100 stars
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wash buffer a smf-wa1-60  (Biosearch Technologies Inc)
90
Biosearch Technologies Inc wash buffer a smf-wa1-60
Phospholipids affect PLIN2 binding to adiposomes Adiposomes were prepared using different amount of PtdIns or DOPE with DOPC in molar ratio. And then, the size of these adiposomes were measured using dynamic light scattering (DLS) (Delsa Nano C Particle Analyzer, Beckman Coulter). After preparation, adiposome preparation (OD600 = 20) was aliquoted equally to 30 μl each and incubated with 1.2 μg PLIN2 per aliquot, supplementing corresponded volume of <t>buffer</t> <t>B</t> to prepare an equal 30 μl specimen. After incubation, the adiposomes were reisolated and washed to remove nonspecific binding proteins. The adiopsome-bound PLIN2 was determined using Western blot with anti-PLIN2 antibody and the density of protein band was quantified by ImageJ. (A) The average diameters of adiposomes with dose of PtdIns (from 0 to 9.6%, mol/mol). (B) The average diameters of adiposomes with dose of DOPE (from 0 to 19%, mol/mol). Inputs of 7.6% of PtdIns and 19% of DOPE were corresponded to the physiological ratio of the two phospholipids on LDs ( Bartz et al. , 2007a ). (C) The binding of PLIN2 on adiposomes with increased doses of PtdIns. (a) The Western blot. (b) The statistical analysis of Western blots using ImageJ. (D) The binding of PLIN2 on adiposomes with increased doses of DOPE. (a) The Western blot of PLIN2. (b) The statistical analysis of Western blots using ImageJ. Data represent mean ± s.e.m., n = 3. ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001, two-tailed t-test. See also <xref ref-type=Figure S2 and . " width="250" height="auto" />
Wash Buffer A Smf Wa1 60, supplied by Biosearch Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wash buffer a smf-wa1-60/product/Biosearch Technologies Inc
Average 90 stars, based on 1 article reviews
wash buffer a smf-wa1-60 - by Bioz Stars, 2026-03
90/100 stars
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buffer b tropix abgs020y  (Tropix Inc)
90
Tropix Inc buffer b tropix abgs020y
Phospholipids affect PLIN2 binding to adiposomes Adiposomes were prepared using different amount of PtdIns or DOPE with DOPC in molar ratio. And then, the size of these adiposomes were measured using dynamic light scattering (DLS) (Delsa Nano C Particle Analyzer, Beckman Coulter). After preparation, adiposome preparation (OD600 = 20) was aliquoted equally to 30 μl each and incubated with 1.2 μg PLIN2 per aliquot, supplementing corresponded volume of <t>buffer</t> <t>B</t> to prepare an equal 30 μl specimen. After incubation, the adiposomes were reisolated and washed to remove nonspecific binding proteins. The adiopsome-bound PLIN2 was determined using Western blot with anti-PLIN2 antibody and the density of protein band was quantified by ImageJ. (A) The average diameters of adiposomes with dose of PtdIns (from 0 to 9.6%, mol/mol). (B) The average diameters of adiposomes with dose of DOPE (from 0 to 19%, mol/mol). Inputs of 7.6% of PtdIns and 19% of DOPE were corresponded to the physiological ratio of the two phospholipids on LDs ( Bartz et al. , 2007a ). (C) The binding of PLIN2 on adiposomes with increased doses of PtdIns. (a) The Western blot. (b) The statistical analysis of Western blots using ImageJ. (D) The binding of PLIN2 on adiposomes with increased doses of DOPE. (a) The Western blot of PLIN2. (b) The statistical analysis of Western blots using ImageJ. Data represent mean ± s.e.m., n = 3. ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001, two-tailed t-test. See also <xref ref-type=Figure S2 and . " width="250" height="auto" />
Buffer B Tropix Abgs020y, supplied by Tropix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/buffer b tropix abgs020y/product/Tropix Inc
Average 90 stars, based on 1 article reviews
buffer b tropix abgs020y - by Bioz Stars, 2026-03
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buffer b  (Ribobio co)
90
Ribobio co buffer b
Phospholipids affect PLIN2 binding to adiposomes Adiposomes were prepared using different amount of PtdIns or DOPE with DOPC in molar ratio. And then, the size of these adiposomes were measured using dynamic light scattering (DLS) (Delsa Nano C Particle Analyzer, Beckman Coulter). After preparation, adiposome preparation (OD600 = 20) was aliquoted equally to 30 μl each and incubated with 1.2 μg PLIN2 per aliquot, supplementing corresponded volume of <t>buffer</t> <t>B</t> to prepare an equal 30 μl specimen. After incubation, the adiposomes were reisolated and washed to remove nonspecific binding proteins. The adiopsome-bound PLIN2 was determined using Western blot with anti-PLIN2 antibody and the density of protein band was quantified by ImageJ. (A) The average diameters of adiposomes with dose of PtdIns (from 0 to 9.6%, mol/mol). (B) The average diameters of adiposomes with dose of DOPE (from 0 to 19%, mol/mol). Inputs of 7.6% of PtdIns and 19% of DOPE were corresponded to the physiological ratio of the two phospholipids on LDs ( Bartz et al. , 2007a ). (C) The binding of PLIN2 on adiposomes with increased doses of PtdIns. (a) The Western blot. (b) The statistical analysis of Western blots using ImageJ. (D) The binding of PLIN2 on adiposomes with increased doses of DOPE. (a) The Western blot of PLIN2. (b) The statistical analysis of Western blots using ImageJ. Data represent mean ± s.e.m., n = 3. ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001, two-tailed t-test. See also <xref ref-type=Figure S2 and . " width="250" height="auto" />
Buffer B, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/buffer b/product/Ribobio co
Average 90 stars, based on 1 article reviews
buffer b - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


Phospholipids affect PLIN2 binding to adiposomes Adiposomes were prepared using different amount of PtdIns or DOPE with DOPC in molar ratio. And then, the size of these adiposomes were measured using dynamic light scattering (DLS) (Delsa Nano C Particle Analyzer, Beckman Coulter). After preparation, adiposome preparation (OD600 = 20) was aliquoted equally to 30 μl each and incubated with 1.2 μg PLIN2 per aliquot, supplementing corresponded volume of buffer B to prepare an equal 30 μl specimen. After incubation, the adiposomes were reisolated and washed to remove nonspecific binding proteins. The adiopsome-bound PLIN2 was determined using Western blot with anti-PLIN2 antibody and the density of protein band was quantified by ImageJ. (A) The average diameters of adiposomes with dose of PtdIns (from 0 to 9.6%, mol/mol). (B) The average diameters of adiposomes with dose of DOPE (from 0 to 19%, mol/mol). Inputs of 7.6% of PtdIns and 19% of DOPE were corresponded to the physiological ratio of the two phospholipids on LDs ( Bartz et al. , 2007a ). (C) The binding of PLIN2 on adiposomes with increased doses of PtdIns. (a) The Western blot. (b) The statistical analysis of Western blots using ImageJ. (D) The binding of PLIN2 on adiposomes with increased doses of DOPE. (a) The Western blot of PLIN2. (b) The statistical analysis of Western blots using ImageJ. Data represent mean ± s.e.m., n = 3. ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001, two-tailed t-test. See also <xref ref-type=Figure S2 and . " width="100%" height="100%">

Journal: iScience

Article Title: Validating an artificial organelle: Studies of lipid droplet-specific proteins on adiposome platform

doi: 10.1016/j.isci.2021.102834

Figure Lengend Snippet: Phospholipids affect PLIN2 binding to adiposomes Adiposomes were prepared using different amount of PtdIns or DOPE with DOPC in molar ratio. And then, the size of these adiposomes were measured using dynamic light scattering (DLS) (Delsa Nano C Particle Analyzer, Beckman Coulter). After preparation, adiposome preparation (OD600 = 20) was aliquoted equally to 30 μl each and incubated with 1.2 μg PLIN2 per aliquot, supplementing corresponded volume of buffer B to prepare an equal 30 μl specimen. After incubation, the adiposomes were reisolated and washed to remove nonspecific binding proteins. The adiopsome-bound PLIN2 was determined using Western blot with anti-PLIN2 antibody and the density of protein band was quantified by ImageJ. (A) The average diameters of adiposomes with dose of PtdIns (from 0 to 9.6%, mol/mol). (B) The average diameters of adiposomes with dose of DOPE (from 0 to 19%, mol/mol). Inputs of 7.6% of PtdIns and 19% of DOPE were corresponded to the physiological ratio of the two phospholipids on LDs ( Bartz et al. , 2007a ). (C) The binding of PLIN2 on adiposomes with increased doses of PtdIns. (a) The Western blot. (b) The statistical analysis of Western blots using ImageJ. (D) The binding of PLIN2 on adiposomes with increased doses of DOPE. (a) The Western blot of PLIN2. (b) The statistical analysis of Western blots using ImageJ. Data represent mean ± s.e.m., n = 3. ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001, two-tailed t-test. See also Figure S2 and .

Article Snippet: The LDs were resuspended using 100 μl Buffer B in each 1.5 ml Eppendorf tube, and washed three times by performing centrifugation at 20,000 g for 5 min at 4°C, to remove other membrane contaminations.

Techniques: Binding Assay, Incubation, Western Blot, Two Tailed Test